Enhancing pesticide degradation using indigenous microorganisms


Diaz, J. et al. Enhancing pesticide degradation using indigenous microorganisms isolated under high pesticide load in bioremediation systems with vermicomposts. 2016. Bioresource Technology. 214: 234-241.



In biobed bioremediation systems (BBSs) with vermicomposts exposed to a high load of pesticides, 6 bacteria and 4 fungus strains were isolated, identified, and investigated to enhance the removal of pesticides. Three different mixtures of BBSs composed of vermicomposts made from greenhouse (GM), olive-mill (OM) and winery (WM) wastes were contaminated, inoculated, and incubated for one month (GMI, OMI and WMI). The inoculums maintenance was evaluated by DGGE and Q-PCR. Pesticides were monitored by HPLC-DAD. The highest bacterial and fungal abundance was observed in WMI and OMI respectively. In WMI, the consortia improved the removal of tebuconazole, metalaxyl, and oxyfluorfen by 1.6-, 3.8-, and 7.7-fold, respectively. The dissipation of oxyfluorfen was also accelerated in OMI, with less than 30% remaining after 30 d. One metabolite for metalaxyl and 4 for oxyfluorfen were identified by GC—MS. The isolates could be suitable to improve the efficiency of bioremediation systems.





This paper shows some practical applications of microbes as bioremediators, exploring the processes of bioremediation in contaminated vericomposts. It explores ways of bioremediation of agricultural waster with microbes.


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